Phytochemical analysis of Portulaca oleracea leaves extract and study the role in protecting genomic human DNA from UV damage
DOI:
https://doi.org/10.56714/bjrs.48.1.1Keywords:
DNA -Protective effects, Portulaca -oleracea L., Oxidative DNA damage, scavenging effectAbstract
Purslane which is the common name for Portulaca oleracea L. is a weed plant distributed all over the world and it has significant medicinal uses due to its present high percentage of phytochemical compounds. However, previous studies by researchers confirmed possession of Purslane leaves extracts anti-oxidant efficacy. Therefore, our study aims to determine the phytochemical composition of the ethanolic leaves extract of purslane and radiation-stimulated DNA damage protecting. Gas chromatography-mass spectrometry (GC-MS) analyses in the present research explore active constituents for P. oleracea, which are 17 active compounds most they are terpenoids and alkaloids. The extract showed considerable antioxidant activity and the highest inhibition-percentage (44.45 %) belongs to (20mg/ml) of an extract with an IC50 value of 4.6 mg/ml in H2O2 scavenging test and prevented DNA oxidative damage stimulated by hydrogen peroxide and ultraviolet light (UV/H2O2) at concentrations of (2-20 mg/mL). These findings suggest that the ethanolic leaves extract of P. oleracea, could be used as skin-care products to prevent UV-induced damage to the skin..
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A. A. Ramos, A. Azqueta, C. Pereira-Wilson, A. R. Collins, J. Agric. Food Chem 58(12), 7465 (2010).
C. A. Juan, J. M. Pérez, F. J. Plou, E. Pérez-Lebeña, Int. J. Mol. Sci 22(9), 464 (2021).
Y. W. Shi, C. P. Wang, X. Wang, Y. L. Zhang, L. Liu, J. Ethnopharmacol 143(3), 896 (2012).
H. P. Lu, Y. N. Jia, Y. Yu, L. Xu, Int. J. Food Prop., 20(sup2) 2207 (2017).
L. Zastrow, N. Groth, F. Klein, D. Kockott, Skin Pharmacol Physiol 22(1), 31 (2009).
K. Jung, M. Seifert, T. Herrling, J. Fuchs, Spectrochimica Acta Part A: Mol. Biomol 69(5), 1423 (2008).
M. A. Salem, M. I. Marzouk, A. M. El-Kazak, Molecules 21(2), 249 (2016).
D. P. Xu,Y. Li, X. Meng, T. Zhou, Y. Zhou, J. Zheng, International journal of molecular sciences 18(1), 96 (2017).
P. Kubatka, S. Uramova, M. Kello, K. Kajo, M. Samec, K. Jasek, D. Büsselberg, Int. J. Mol. Sci 20(7), 1749 (2019).
F. Zhu, B. Du, B. Xu, Crit Rev Food 58(8), 1260 (2018).
S. Petropoulos, A. Karkanis, N. Martins, I. Ferreira, Trends Food Sci Technol 55(1), 10 (2016).
V. Sicari, M. R. Loizzo, R. Tundis, A. Mincione, T. Pellicano, J. Appl. Bot. Food Qual 91(39), 5073 (2018).
D. M. Aziz, S. G. Taher, J. R. Hama, MOJ Food process Technol 2(4), 00047 (2016).
S. R. Al-Mosawi, H. M. Al-Saily, Ann Rom Soc Cell Biol 2622, 1365 (2021).
K. Verma, D. Shrivastava, G. Kumar, J. Taibah Univ. Sci. J 9(1), 34 (2015).
M. B. Adinortey, C. Ansah, A. Weremfo, C. A. Adinortey, G. E. Adukpo, E. O. Ameyaw, A. K. Nyarko, J Nat Sci Biol Med 9(1), 6 (2018).
K. Bekhouche, T. Ozen, S. Boussaha, S. Koldas, S. Yenigun, S. Lassed, D. Zama, Bangladesh J. Pharmacol 13(1), 82 (2018).
S. C. Okereke, I. I. Ijeh, U. O. Arunsi, AJPP 11(2), 25 (2017).
M. G. Hegazy, A. M. Imam, B. E. Abdelghany, Tumor Biol 42(5), 1177 (2020).
R. Shah, P. K. Verma, Chemistry central journal 12(1), 1 (2018).
R. Gudipati, R. N. Reddy, S. Ma, J Enzyme Inhib Med Chem 26(6), 813 (2011).
A. O. Hussein, G. I. Mohammed, M. Y. Hadi, I. H. Hameed, J. Pharmacogn. Phytotherapy 8(3), 49 (2016).
J. Wang, Y. Pang, W. Jing, W. Chen, R. Guo, X. Han, N. Chu, Infect Drug Resist 12, 763 (2019).
B. M. Sumathi, F. Uthayakumari, JSRR 4(1), 24 (2014).
M. A. Musa, V. L. Badisa, L. M. Latinwo, Anticancer Res 34(4), 1601 (2014).
K. Fujiwara, H. Kawamura, H. Hirano, K. Takahashi, T. Maeda, M. Kolke, In Corrosion97. OnePetro (1997).
J. Feng, M. Weitner, W. Shi, S. Zhang, D. Sullivan, Y. Zhang, Antibiotics 4(3), 397 (2015).
M. F. Ahmad, M. O. Ansari, N. Parveen, S. Ahmad, S. Jameel, G. Shadab, World J. Pharma. Res 5(1504), 42 (2016).
D. Tungmunnithum, A. Thongboonyou, A. Pholboon, A. Yangsabai, An overview. Medicines 5(3), 93 (2018).
M. B. Majnooni, S. Fakhri, G. Bahrami, M. Naseri, M. H. Farzaei, J. Echeverría, Evid Based Complement Alternat Med 21, 10 (2021).
M. Alam, A. S. Juraimi, M. Y. Rafii, A. Abdul Hamid, A. Aslani, BioMed research international (2014).
S. R. Al-Mosawi, H. M. Al-Saily, Ann Rom Soc Cell Biol 2622, 1365 (2021).
E. Proshkina, S. Plyusnin, T. Babak, E. Lashmanova, F. Maganova, L. Koval, A. Moskalev, Antioxidants 9(6), 529 (2020).
M. Yu, I. Gouvinhas, J. Rocha, A. Barros, Sci. Rep 11(1), 1 (2021).
M. M. El-Zayat, M. M. Eraqi, H. Alrefai, A. Y. El-Khateeb, M. A. Ibrahim, H. Aljohani , M. M. Elshaer, Biomolecules 11(3), 470 (2021).
T. P. Durgawale, C. C. Khanwelkar, P. D. Pratik, Asian J Pharm Clin Res 11 (9) , 7 (2018).
F. Collin, Int. J. Mol. Sci 20(10), 2407 (2019).
G. Guha, V. Rajkumar, L. Mathew, R. A. Kumar, J. Biol 35(2), 233 (2011).
V. Reddy, A. Urooj, A. Kumar, Food Chem 90(2), 317 (2005).
P. Kubatka, S. Uramova, M. Kello, K. Kajo, M. Samec, K. Jasek, D. Büsselberg, Int. J. Mol. Sci 20(7), 1749 (2019).
N. Kushwaha, D. Mondal, V. K. Gupta, M. V. Jithin, J. Pharmacogn. Phytochem 6(3), 123 (2017).
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