Upregulation of ABL1 Gene Expression Due to the Co-expression of PML-II and adenovirus Genome Transfection
DOI:
https://doi.org/10.56714/bjrs.48.1.8Keywords:
Adenovirus type 5, PML gene, Transform cells, apoptosis, Tumor suppressor gene, oncogenic protein, TransfectionAbstract
Several promyelocytic leukemia proteins (PML) isoforms are involved in a wide range of cellular functions, including innate immune responses, gene transcription, and apoptosis. Adenoviridae are non-enveloped viruses that can disrupt cellular systems that control cell cycle progression and apoptosis,. The study analyzed the gene expression of ABL-1(Tyrosine Kinase gene), into cells transfected with adenovirus and PML-II expression plasmids. A human breast cancer cell line (MCF-7) cells were plated in 24 well plates in 500 microliters of Dulbecco's Modified Eagle Medium (DMEM) for each well, and after 24 hours, the cells were harvested and used for gene expression analysis. So, the aim was evaluate the effect of the effect of PML-II on ABL1 gene expression.
The results showed that ABL1 gene expression was in transfected Ad5 and PML samples compared to the control.
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