Molecular study of Toxoplasma gondii infection in normal delivery women with previous abortion history at Basrah Province
DOI:
https://doi.org/10.56714/bjrs.48.2.14Keywords:
Toxoplasma gondii, Recurrent abortion Molecular study, Nested PCRAbstract
Toxoplasma gondii is the causative agent of toxoplasmosis, one of the most common parasitic infections of humans and pets. T. gondii was serologically investigated for newborn women who had previous abortion by ELISA and included (70) women with a history of single or recurrent abortion and (30) women with normal delivery without previous abortion as a control group. The study included women attending Basrah Obstetrics and Gynecology Hospital, Al-Moanaa General Hospital, Al-Fayhaa General Hospital, Zubair General Hospital and Dar Al Shifa investment Hospital. The percentage of seroprevalence of T. gondii IgG was (41.4%). No percentage was recorded for IgM antibodies. Significant differences were found between age groups of these antibodies. The examination showed that 7 (23.3%) of (30) in the control group were positive for IgG antibodies, compared to 0 (0%) of IgM. The highest percentage (50%) of IgG antibody was in the age group 20 years or less according to the number of previous miscarriages. The highest percentage of IgG antibody infection was (46.2%) in women who had two previous abortions and no infection rates were recorded for IgM antibody. The highest infection rates (43.8%) were in Zubair district compared to the city center for IgG antibody, and no infection with IgM antibody was recorded. Infection rate (70%) was recorded for the PCR-positive samples using Nested PCR technique. Toxoplasma DNA sequences according to the isolated hosts was carried out. The reading of the genetic tree showed that the sample of women born naturally is identical with the main branch of the kinship tree that carries the sequence MZ717187, MZ717188, MZ717189, MZ717190, MZ717191 in the global gene bank.
Downloads
References
D.J.P. Ferguson, Memorias do Instituto Oswaldo Cruz 104(2), 133 (2009).
A. Al-Tamemmi, B.H. Abdullah, S. J. Raisan. European Journal of Biomedical and Pharmaceutical Sciences 6(1), 99 (2019).
T. Ali , D.S. Mahdi and A.H. Awad. International Journal of Advanced Research 7(1), 629 (2019).
S.M. Nourollahpour, A. Rostami, B.D. Pearce, M.M. Gholipourmalekabadi, D.J. Newport, M. Danesh, S. Mehravar, S.J. Seyyedtabaei, European Journal of Clinical Microbiology and Infectious Diseases 35,1829 (2016).
E. Yousefi, M. Foroutan, R. Salehi,S. Khademvatan, Journal of Acute Disease 6(3),120 (2017).
S. Fallahi, A. Rostami, M.N. Shiadeh, H. Behniafar,S. Paktinat, Journal of Gynecology Obstetrics and Human Reproduction 47(3), 133 (2018).
A. Rostami, H. Keshavarz, S. Shojaee, M. Mohebali, A.R. Meamar, Iranian Journal of Parasitology 9(4), 474 (2014).
S. Khademvatan, N. Khajeddin, J. Saki, S. Izadi-Mazidi, African Journal of Science 109(1), 1 (2013)
S. Khademvatan, F. Riahi, M. Izadi-Mazidi, N. Khajeddin, N. Khajeddin, E. Yousefi, The Pediatric Infectious Disease Journal 37(11), 1097 (2018).
W. Shani, “Humoral and cellular immune response in women afflicted with Toxoplasmosis,” Ph. D. thesis. College Of Science, Basrah University, Basrah, (2004).
C. Contini, S. Seraceni, R. Cultrera, C. Incorvaia, A. Sebastiani,S. Picot,. International Journal for Parasitology 35(3), 275 (2005).
P. Bastien, E. Jumas-Bilak, E. Varlet-Marie, P. Marty, Clinical Microbiology and Infection 13(4), 430 (2007).
J. Jones, M.D., M.P.H., A. Lopez, M.H.S., M. Wilson, M.S, Congenital toxoplasmosis. American Family Physician 67(10), 2131 (2003).
M.M. Al-Hamdani, N.K. Mahdi, Eastern Mediterranean Health Journal 3(2), 310 (1996).
N. Roizen, C.N. Swisher, M.A. Stein, J. Hopkins, K. M. Boyer, E. Holfels, M.B. Mets, L. Stein, D. Patel, P. Meier, et al., Neurologic and developmental outcome in treated congenital toxoplasmosis 95(1), 11 (1995).
R.G. Al-Simani, “Study of detection and diagnosis of toxoplasmosis in the center of Ninevah Governorate,” M.S. thesis, College of Veterinary Medicine, Mosul University, Mosul (2000).
Z.A.A. Al-Rubaia, “Comparative between enzyme linked immunoserbent assay and enzyme
linked fluorescent assay diagnosis of Toxoplasma gondii in pregnant women and its relationship with abortion cases and abnormalities in Diwaniya province,” M. S. thesis. College of science, Al-Qadisiya University, Al-Qadisiya (2008).
S.J. Al-Ghezy, “Diagnostic study of Toxoplasma gondii and Cytomegalovirus in pregnant and aborted Women with some Epidemiological and Immunty parametery in Thi-Qar province- Iraq,” M. S. thesis College of Education of pure science. Thi-Qar University, Thi-Qar (2012).
B.J. Freij, J.L. Sever, Pediatrics In Review 12(8), 227 (1991).
A.A. Ali, “Relation of zinc and copper levels with some immunological markers in women with toxoplasmosis in Kikuk governorate with trail to treatment in mice,” Ph. D. Thesis, College of Education, Tikrit University. Tikrit (2008).
B.D. A. AL- Doski, “Seroepidemiological study of toxoplasmosis among different groups of population in Duhok city by using Latex agglutination test and indirect hemagglutination test,” M.S. thesis, College of Medicine, Duhok University, Duhok (2000).
N.F. Ageel, “Serological and biochemical study of toxoplasmosis in Tikrit teaching Hospital,” M.S. thesis, College of Medicine, Tikrit University, Tikrit (2003).
M.K. Al-Maqdisy, “A survey study of the aborted cases in sheep and human caused by Toxoplasma gondii in Nineveh Governorate,”M .S. thesis, College of Veterinary Medicine, Mosul University, Mosul (2000).
K.Y. Al-Dugaily, “A seroepidemiological study of toxoplasmosis among aborted women in Baghdad,” M.S. thesis, College of Science, Baghdad University, Baghdad (1998).
J.G. Montoya, O. Liesenfeld, S. Kinney, C. Press, J. S. Remington, Journal of Clinical Microbiology 40(7), 2504 (2002).
R.F. Al-Kalaby, “Sero-epidemiological study of toxoplasmosis among different groups of population in Najaf city,” M. S. thesis. College of Medicine, Kufa University, Kufa (2008).
R.K. Al-Abudy, “Diagnostic and Epidemiological study of Toxoplasma gondii and Rubella virus in aborted women by technology Real-Time PCR in Thi-Qar province-Iraq,” M. S. thesis College of Education of pure science, Thi-Qar University, , Thi-Qar (2014).
D.J.P. Ferguson, S.F. Parmley, S. Tomavo, International Journal for Parasitology 32(11), 1399 (2002).
S.K. Al-Hadraawy, F.A. Hadi, International Journal of Pharmacognosy and Phytochemical Research 9(4), 482 (2017).
Hurtado, G. Aduriz , B. Moreno, J. Barandika, A.L. Garcia-Perez, Veterinary Parasitology 102(1-2), 17(2001).
E. Chabbert, L. Lachaud, L. Crobu, P. Bastien, Journal of Clinical Microbiology 42(4), 1719 (2004).
D. G. Mordue, N. Desai, M. Dustin, and L.D. Sibley, Journal of Experimental Medicine 190(12), 1783 (1999).
E.J.C. Goldstein, J.G. Montoya, S. Remington, Clinical Infectious Diseases 47(4), 554 (2008).
S.H. Mahmood, H.H. Hassani, K.H. Zghair, Iraqi Journal of Medical Sciences 8(3), 42 (2010).
C. Andrei, Analele University din Oradea, Fascicula: Ecotoxicologie, Zootehnie și Tehnologii de Industrie Alimentara 1(3-5), 261 (2010).
R. Thiebaut, S. Leproust, G. Chene, R. Gilbert, A meta-analysis of individual patients data 369(9556), 115 (2007).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 College of Education for Pure Science, University of Basrah
This work is licensed under a Creative Commons Attribution 4.0 International License.
