Molecular Detection of Protozoal Infection in Diarrheic Children in Wasit Province
Keywords:
diarrhea in children, intestinal parasite, conventional PCRAbstract
This cross-sectional study was conducted in Wasit province. collected 296 stool sample diarrheic children less than 16 years, selected 96 Randomly from some patients in hospitals and public health centers in Al-kut city and private clinics (from 1st December 2023 to 1st May, 2024 were included in the study. Method Finding the form of the parasite through direct smear microscopy to confirm the presence of an intestinal parasite infection, and diagnosis this parasite by molecular method polymerase chain reaction technique (PCR). The results showed that the cases were matched by age, gender, and where the person lived. The diarrhea was looked at using direct smear microscopy, which showed that Entamoeba histolytica, Giardia.lamblia, and Cryptosporidium parvum were found 23(24.0%), 5(5.2%) and 4(4.2%) respectively. While, Diagnosis by polymerase chain reaction(PCR). This result was 64(66.67%), 11(11.46%), 23(23.96%), respectively. In conclusion, PCR assay of molecular detection to protozoal infection in the present study is more accuracy than traditional methods like direct smear
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